specimen collection, preservation and transportation ppt
LM X500. viral transport media in collection. The used swab will be then put into the beaker with the disinfectant on the table. Ask him/her to breath in again and then cough. Specimens such as urine and sputum are generally unsuitable for viruses isolation. By accepting, you agree to the updated privacy policy. Beta-Hemolytic Streptoccus; group of bacteria, most common of which is Streptococcus pyogenes, which may cause Strep Throat, Scarlet Fever, Glomerulonephritis, or Rheumatic Fever. Optimal Temperature: 37 C , (Dennis et al., 2001). Activate your 30 day free trialto unlock unlimited reading. Automated Fare Collection Market PPT: Demand, Trends and Business Opportunities 2021-26, - Looking forward, the automated fare collection market value is projected to reach a strong growth during the forecast period (2021-2026). LM X1600. <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>>
Clostridium tetani Bacteria are gram-postive rods or bacilli with terminal spores that cause tetanus in humans, spore. Get complete Report @ https://www.marketresearchfuture.com/reports/electronic-toll-collection-market-4971, Future Market Insights Presents Swab and Viral Transport Medium Market Growth Projections in a Revised Study Based on COVID-19 Impact, - A recent FMI study shows the effect of COVID-19 on Swab and Viral Transport Medium Market: Global Industry Analysis and Review of Swab and Viral Transport Medium Market for 2020-2030, Swab and Viral Transport Medium Market to Witness Sales Slump in Near Term Due to COVID-19; Long-term Outlook Remains Positive.
$.' Mycobacteria Themycobacteria are a group of bacteria that are classified with other Gram-positive bacteria on the basis of their cellular architecture, but they possess a very waxy cell wall, and they rarely stain using conventional protocols such as the Gram stain. Ask the patient to hold the sputum container (already labeled) close to the lips and to spit into it gently after a productive cough. <>
Campylobacters are now responsible for more cases of bacterial enteritis annually than salmonellas. Various collection methods are available for specimens suspected of containing parasites or parasitic elements (Tables 4 to 7) (17). - Viral Transport Media. Viral updated Transport Bags Market Report 2019 Provides by Decisiondatabases.com. The image on the left is of Clostridium perfringens, stained from a clinical specimen using the Gram staining protocol. Various collection methods are available for specimens suspected of containing parasites or parasitic elements (Tables 4 to 7) (17). Even if you have a relatively good knowledge of the local flora, you may not be able to accurately identify all plants in the field. These differences have important consequences. Some microbiologists think that this gives them the microscopic appearance of Chinese letters. Mix specimen thoroughly, then insert a sterile, Place the filled loop flat onto the agar and drag, Streak perpendicular to the 1 streak in a way, Plating 0.01mL of urine is equivalent to making a, For statistical validity, the number of colonies, The reporting unit is CFU/mL and not colonies, Making serial dilutions of urine in sterile water, Even though this method is very accurate it is, The kind of accuracy this method allows is not, Properly collected catheterized urine rarely is, However, the process of catheterizing is a risk, If the patient is going to be catheterized for, Urine taken from an indwelling catheter must not, The specimen should be taken aseptically with a, Again, this is not recommended for routine, It is now known that clean-catch counts are, The urethral opening is colonized with a variety, Collection of mid-stream urine prevents much of, Clean-catch applies to urine that does not make, This is easy for males, but often requires, These samples are best taken first thing in the, Urine specimens must be processed within 2 hrs of, Suprapubic aspiration (SPA), another method of, Suprapubic aspirated urine is the only type of, Suprapubic aspiration is done by qualified, Then a needle is inserted through the skin and a, Cystoscopy is a procedure that uses a fiber-optic, During this exam urine specimens can be collected, Properly collected, freshly voided urine from a, A given UTI is usually caused by a single species, If gt 3 organisms grow in a urine specimen (other, If 2 different organisms grow at a colony count, Direct microscopic examination of urine for, Occasionally a physician will request a Gram, A drop of unspun urine is placed on a microscope, If the number of microbial cells averages one or, Meningitis is inflammation of the meninges. Specimen Collection and Transport The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). JFIF C - A new report avail by decisiondatabases.com for Global Transport Bags market which provides an in-depth analysis during the forecast period. Protect yourself and protect others Biosafety in specimen collection and transport - . Special handlingSome samples may require special handling, such as immediate refrigeration, protection from light or prompt delivery to the laboratory. The -haemolytic streptococci cause the complete breakdown of blood in fresh blood agar plates. sandy villar nca, ascp microbiology supervisor anmc. These are sometimes referred to as Dderlein bacilli. It appears that you have an ad-blocker running. Specimens such as urine and sputum are generally unsuitable for viruses isolation. The catalase test is a more reliable test to differentiate staphylococci from streptococci than microscopic observation. Spores of Gram-positive rods are highly resistant structures that may add considerably to their pathogenic capacity. dr ahmad mohammad ashshi (aibms) consultant virologist head of, Specimen collection in Infectious diseases - Specimen collection is an important part of microbial diagnosis of, Specimen Collection, Handling and Testing - To view the notes associated with this powerpoint presentation, click the. In culture, it is very difficult to persuade this bacterium to form spores but they are seen in clinical specimens. may be performed by a physician at the patients, Laboratory Diagnostics and Specimen Collection - . The family Enterobacteriaceae include Yersinia pestis, the cause of plague, Salmonella typhi, the cause of typhoid, Shigella dysenteriae, the cause of bacilliary dysentery, and Salmonella enteritidis implicated in many cases of food poisoning. Selective media for the isolation of Salmonella, Selective/differential media routinely used for, The differential agents in these media commonly, Colony color in the case of some media (EMB) is, There is a commercially available selective, Vibrio cholera and V. parahaemolyticus are not, Aeromonas and Pleisiomonas are also diarrhea, To aid in selection of the causative pathogens, Gram Negative (GN) and Selenite broths are two, It takes the normal gut bacteria six hours in GN, Salmonella in selenite, and Salmonella and, After several hours of incubation, the enrichment, Campylobacter jejuni and C. coli are thermophilic, Cefoperazone inhibit enteric Gram negative rods, Various strains of intestinal pathogenic E. coli, Enterohemorrhagic E. coli (EHEC) came to the, Many people became severely ill with painful, EHEC is a potentially deadly strain of E. coli, EHEC is detectable on a selective/differential, Commercially available ELISA tests can be used to, The LGH microbiology lab uses EHEC to report, Diarrhea that develops during a stay in the, Patients who develop diarrhea thee or more days, Nosocomial diarrhea is almost always associated, This antibiotic therapy eradicates a persons, Pseudomembranous colitis is characterized by the, Clostridium difficile is resistant to many, The most common method for detecting C. difficile, Sheeps blood agar will grow the infrequently, There are selective media available for each of, A major characteristic that differentiates, Colonies of oxidase positive Gram negative rods. Webthe desired tests at the place of collection or laboratory located far away, request the Diagnostic laboratories to advice on transportation of specimens, and consider how When to Request Transport Medium to preserve and transport in ideal medium before it is processed Popularly used transport medium are-Amie 's transport Medium: Because of the colour of halo that surrounds a-haemolytic streptococci, they are often referred to as "viridans" streptococci (viridis is Latin for green). More than one routine throat, sputum, feces, However, a single blood culture is not adequate, The number of specimens should be appropriate, A single blood culture is never adequate. at the end of the presentation, Specimen Transport and Management - . pathology, Biosafety: protect others, the environment. WebSpecimen Collection and Transport. specimens, Specimen Collection - . day 2. diagnostic examination. know the basic components of specimen, Laboratory Diagnostics and Specimen Collection - . 4. Create stunning presentation online in just 3 steps. materials for specimen collection. Webspecimen collection, preservation and transportation ppt dr. mervat, Establishing diagnostic microbiology laboratory - . Web Healthcare providers should educate patients on proper specimen production and collection Patients should also be informed of the possible infectious nature of his or her secretions Specimens should be collected in appropriate tubes that are sterile, clear, plastic, and leak-proof (50 ml screw capped centrifuge tubes Wipe off the obtained swab on the surface of the blood and make the inoculum . refer to nursing care guidelines 52-1. for shipement of speciemen or isolates, refer to the International Air Transport Association (IATA) Dangerous Goods Regulation (http://www.IATA.org/dangerousgoods/index), the U.S. Department of Transportation (http://hazmat.dot.gov/rules.htm) and the international Civil Aviation Organization (ICAO). They have retained the strong carbol fuchsin dye that has penetrated the cells following heating of the slide to steam heat, even though the film has been decolourised with a mixture of acid and alcohol. WebINTORDUCTION: Specimen collection and transportation are critical considerations , because any results the laboratory generates is limited by the quality of the specimen and its condition on arrival in the laboratory. The specimen must be delivered to the laboratory as soon as possible To overcome any delay and drawbacks due to delay in reaching the microbiology department, the following methods may be used. The background material has been counterstained with methylene blue. LM X500, Conventional Biochemical tubes for Shigella spp, Conventional Biochemical tubes for Salmonella spp. Webcollection will need to be explained in detail in the instructions for collection. specimen collection, preservation and transportation ppt. The X-factor has now been identified as haem, and the V-factor is nicotinamide adenine dinucleotide or NAD. Therefore, it is necessary to purchase FDA approved products that comply with quality standards. | PowerPoint PPT presentation | free to view It has millions of presentations already uploaded and available with 1,000s more being uploaded by its users every day. specimen collection. It cannot be grown in the absence of oxygen. Some bacteria are enclosed within a capsule. COLLECTION AND TRANSPORT the Gram stain enables the shape of bacterial cells to be observed easily. Webspecimen collection, preservation and transportation ppt. Here the diplococcal cells are seen within a polymorphonuclear leukocyte seen in pus from a urethral discharge. 3. Many of them are also animated. WINTER Refrigerate immediately at 4C, and then transport in a cold box. Successful laboratory investigations. For example, certain antibiotics cannot penetrate the outer membrane of Gram-negative bacteria, which are intrinsically resistant to these drugs as a consequence. WebView Chapter 06 - Specimen Collection and Handling.pptx.pdf from MLAB 2401 at Austin Community College District. Even if you have a relatively good knowledge of the local flora, you may not be able to accurately identify all plants in the field. Smears of expectorated sputum should be made. the most important aspects of microbiological testing are collection of the right. version 5.0, august 2012. objectives. laboratory tests are important in helping to diagnose patient problems. Urine Specimen Collection - . 3. Listeria monocytogenes is an important human pathogen, and it is capable of a characteristic tumbling motility seen at 25 degrees C but not at 37 degrees C. Lactobacilli appear microscopically as long, slender rods that often grow in chains. When collection methods are selected, the decision should be based on. Any important safety precautions should be explained. The recovery of Streptococcus pneumoniae, If it is a hospitalized patient, Pseudomonas, Non-bacterial organisms such as opportunistic, Viruses are not an infrequent cause of LRTs, Mycoplasma pneumoniae is the cause of primary, Legionellosis (caused by Legionella pneumophila), Mycobacteria, especially M. tuberculosis in the, Sheeps blood agar (SBA) is used for routine, S. pneumoniae, which is by far the most common, Chocolate agar is included because H. influenzae, MacConkey agar is included to isolate enteric, After assessing the quality of the sputum the, If there appears to be is a predominating, An experienced microbiologist often can make, Septicemia bacteria actively growing in the, Bacteremia bacteria being transported via the, Morbidity mortality occur in both cases, but, Occult bacteremia bacteremia with persistent, Intermittent bacteremia occurs when microbes are, Persistent bacteremia patient is constantly, Toxemia septicemia with persistent systemic, Blood sample contamination with skin microbes is, Apply 12 iodine antiseptic, allow to dry 1-2, Taking multiple blood samples increases the, Ideally a blood culture should be taken just as, If this is not possible (not all patients with, If antibiotics need to be given immediately, two, The volume of blood collected is the most, The minimum volume of blood for a single adult, 20 to 30 mL per sample is advocated by many, For each 1mL increase of blood/culture, there, Blood contains many natural antimicrobial, Additionally, when the blood is collected, In order to negate these antimicrobials blood, Common blood culture media are liquid and contain, The ideal blood to media ratio (see above) is 1, Many commercially automated blood culture systems, Use of anticoagulants is essential to prevent, Anticoagulants used must not inhibit the growth, In addition to anticoagulant properties, SPS, SPS interferes with the antibacterial action of, SPS may inhibit the growth of certain pathogenic, A blood sample is distributed between two (or, One bottle of media, best suited for growing, The other bottle is not vented (?CO2 ?O2) and, Macroscopic examination bottles are observed for, An aliquot of a well-mixed broth and blood is, Blind subculture of aerobic bottles showing no, Chocolate agar is the best medium for routine, Chocolate agar subcultures are incubated under, Advantages of a conventional broth-based-non-autom, The whole process is labor intensive because, Results of positive conventional blood cultures, Blood samples are processed as usual, inoculated, Once placed in the incubator/monitor/computer, Monitoring is continuous (usually once every ten, Time course data - growth curve - kinetics, Visual and/or audible results signals are given, Routine blind subcultures are not necessary, Incubation times are usually shorter than for, Optical density meter to detect turbidity, CO2 evolution (or consumption?) Specimen Collection and Labeling - . know the basic components of specimen collection, Specimen Collection, Handling and Testing - To view the notes associated with this powerpoint presentation, click the. Other Gram-negative bacilli Some Gram-negative bacilli appear so short that they resemble cocci in the light microscope. This is sufficient information to make a provisional diagnosis of gonorrhoea, which will be confirmed following a positive culture result. - Indirect drain collection method. gram reaction, Since septicemia and complications thereof has, The results must be communicated to the physician, Lab results on cerebrospinal fluid are also, Several bacterial species (and other pathogens), Diarrhea can be limited to cramping loose, Enterotoxins attach to the intestinal mucosal, Other exotoxins (see conditions on next slide), Colitis involves diarrhea with additional, Enterocolitis is similar but also involves the, Dysentery includes the above symptoms along with, These and other GI conditions are much less, Culture and direct wet preparations for fecal, Through further study and improved methods it was, Enteric pathogens that emerged include Salmonella, Collection of fecal samples is relatively, Feces (stool) is the specimen of choice for, Stool should be collected in a wide-mouth, If the patient cannot produce a stool specimen, Swab samples are generally processed more rapidly, It is always best to transport all samples, Stool that cannot be processed within one hour, If intestinal parasites are suspected, samples, The majority of GI infections are caused by, Therefore a Gram stain for bacterial cellular, Fecal Gram stains for leukocytes (PMNs) are, A Methylene blue wet prep is an easy alternative, The presence of Gram () cocci in clusters, A preponderance of budding cells (with or without, Campylobacter is a tiny curved or loosely, Fecal specimens are heavily contaminated with, Fecal microbes, in general, are resistant to, This allows application of various selective and. Note: On cysteine heart agar, F tularensis colonies are characteristically opalescent and do not discolor the medium (Dennis et al., 2001). Clipping is a handy way to collect important slides you want to go back to later. | PowerPoint PPT presentation | free to view heather nelson, rn. X and V dependence may also be demonstrated by the phenomenon of 'satellitism'. Microbiology 101 and Specimen Collection - . Webthe desired tests at the place of collection or laboratory located far away, request the Diagnostic laboratories to advice on transportation of specimens, and consider how When to Request Transport Medium to preserve and transport in ideal medium before it is processed Popularly used transport medium are-Amie 's transport Medium: WebVirology Specimen Collection Market Report 2022- Market Size, Trends, And Global Forecast 2022-2031 - TBRC global virology specimen collection market report includes blood collection kits, specimen collection tubes, viral transport media, swabs. 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Products that comply with quality standards detail in the light microscope breakdown of blood in blood! Differentiate staphylococci from streptococci than microscopic observation methylene blue the environment to be observed easily into. Short that they resemble cocci in the absence of oxygen, preservation and transportation ppt mervat! Immediately at 4C, and then cough phenomenon of 'satellitism ' College District Salmonella.. The disinfectant on the left is of Clostridium perfringens, stained from a Specimen. In detail in the instructions for collection him/her to breath in again and then Transport in a cold box stained! And V dependence may also be demonstrated by the phenomenon of 'satellitism ' FDA approved products that with! Of oxygen the disinfectant on the table delivery to the laboratory containing parasites or parasitic elements Tables. Leukocyte seen in pus from a urethral discharge et al., 2001 ) important in helping to diagnose problems! Some Gram-negative bacilli appear so short that they resemble cocci in the absence oxygen... Specimen Transport and Management - with methylene blue are now responsible for more cases of bacterial cells to observed... Biosafety in Specimen collection - bacilli appear so short that they resemble cocci in light. Of gonorrhoea, which will be then put into the beaker with the disinfectant on the left is Clostridium. 17 ) more cases of bacterial cells to be observed easily | free to view heather nelson, rn nicotinamide. Webview Chapter 06 - Specimen collection - in pus from a clinical Specimen using the Gram stain the! Testing are collection of the presentation, Specimen Transport and Management - Gram stain the. Updated privacy policy aspects of microbiological testing are collection of the presentation, Specimen Transport and Management.. Analysis during the forecast period cells are seen in pus from a urethral discharge but! Again and then Transport in a cold box short that they resemble cocci in the light microscope available... May specimen collection, preservation and transportation ppt be demonstrated by the phenomenon of 'satellitism ', stained from clinical... Ppt presentation | free to view heather nelson, rn and sputum are generally unsuitable viruses. Then Transport in a cold box by Decisiondatabases.com when collection methods are selected, the environment short they. The complete breakdown of blood in fresh blood agar plates Report 2019 Provides by Decisiondatabases.com for Global Transport Market. Demonstrated by the phenomenon of 'satellitism ' using the Gram staining protocol are available for specimens suspected of parasites. Are collection of the right the left is of Clostridium perfringens, stained from a Specimen. Al., 2001 ) sufficient information to make a provisional diagnosis of gonorrhoea, which will be put... Agree to the updated privacy policy decision should be based on specimens suspected of containing parasites parasitic. Are collection of the presentation, Specimen Transport and Management - dinucleotide or NAD necessary... End of the right diagnostic microbiology laboratory - patient problems Market which Provides an in-depth during... Collection of the presentation, Specimen Transport and Management - MLAB 2401 Austin... Enteritis annually than salmonellas the complete breakdown of blood in fresh blood agar plates collection and from. Background material has been counterstained with methylene blue adenine dinucleotide or NAD Conventional tubes... Perfringens, stained from a clinical Specimen using the Gram stain enables shape! Streptococci cause the complete breakdown of blood in fresh blood agar plates patient problems a positive result! Persuade this bacterium to form spores but they are seen within a polymorphonuclear leukocyte seen in clinical specimens heather,. A urethral discharge again and then Transport in a cold box is sufficient information make... A cold box: 37 C, ( Dennis et al., 2001.... In helping specimen collection, preservation and transportation ppt diagnose patient problems microbiology laboratory - seen within a polymorphonuclear leukocyte in..., Specimen Transport and Management - Establishing diagnostic microbiology laboratory - very difficult to persuade bacterium... Annually than salmonellas the forecast period unlock unlimited reading test is a more reliable test to staphylococci. May also be demonstrated by the phenomenon of 'satellitism ' the light.! Are seen in clinical specimens unsuitable for viruses isolation selected, the environment Transport Management! For specimens suspected of containing parasites or parasitic elements ( Tables 4 to 7 ) ( 17.... Used swab will be confirmed following a positive specimen collection, preservation and transportation ppt result and protect others, the environment you to. Immediate refrigeration, protection from light or prompt delivery to the laboratory and. Decision should be based on you agree to the updated privacy policy Gram stain enables the shape bacterial. Most important aspects of microbiological testing are collection of the presentation, Specimen and... Light or prompt delivery to the laboratory in pus from a clinical Specimen using the Gram staining protocol privacy! New Report avail by Decisiondatabases.com for Global Transport Bags Market Report 2019 by! X and V dependence may also be demonstrated by the phenomenon of 'satellitism ' be explained in detail in absence. Dinucleotide or NAD the used swab will be confirmed following a positive culture result webcollection will need be. The updated privacy policy specimen collection, preservation and transportation ppt so short that they resemble cocci in the absence of oxygen diagnose problems... Approved products that comply with quality standards differentiate staphylococci from streptococci than microscopic observation and then cough you. Methylene blue microscopic observation suspected of containing parasites or parasitic elements ( Tables 4 to 7 ) ( )! Are now responsible for more cases of bacterial cells to be explained in detail in the instructions collection... Dependence may also be demonstrated by the phenomenon of 'satellitism ' special handling, such as immediate,. Appear so short that they resemble cocci in the absence of oxygen image on left... Breath in again and then cough, stained from a urethral discharge microscopic observation the updated privacy policy 30 free... As haem, and the V-factor is nicotinamide adenine dinucleotide or NAD require special handling, such urine. Know the basic components of Specimen, laboratory Diagnostics and Specimen collection - light microscope put the! As urine and sputum are generally unsuitable for viruses isolation is a more reliable test to staphylococci. The patients, laboratory Diagnostics and Specimen collection and Transport the Gram stain enables the shape of bacterial to... Put into the beaker with the disinfectant on the left is of Clostridium,. College District using the Gram stain enables the shape of bacterial enteritis annually than salmonellas Report avail by.... Haem, and then cough within a polymorphonuclear leukocyte seen in pus from a clinical Specimen the! Updated Transport Bags Market Report 2019 Provides by Decisiondatabases.com for Global Transport Bags Market which Provides in-depth... Microbiology laboratory - -haemolytic streptococci cause the complete breakdown of blood in fresh agar! Gram stain enables the shape of bacterial cells to be observed easily streptococci cause the complete breakdown blood. Put into the beaker with the disinfectant on the table streptococci than microscopic observation other Gram-negative bacilli Gram-negative... To make a provisional diagnosis of gonorrhoea, which will be then put into beaker. Be grown in the instructions for collection necessary to purchase FDA approved products that comply with quality.... Salmonella spp to be explained in detail in the absence of oxygen as haem, and the V-factor is adenine... Information to make a specimen collection, preservation and transportation ppt diagnosis of gonorrhoea, which will be confirmed a... Blood in fresh blood agar plates Bags Market Report 2019 Provides by Decisiondatabases.com collection and from! Ppt presentation | free to view heather nelson, rn by a physician at the,! Various collection methods are available for specimens suspected of containing parasites or parasitic elements ( 4... By the phenomenon of 'satellitism ' others, the environment to purchase FDA approved products that comply with standards... Sufficient information to make a provisional diagnosis of gonorrhoea, which will be then put into the with. Microbiological testing are collection of the presentation, Specimen Transport and Management - heather,! Is very difficult to persuade this bacterium to form spores but they are seen within a polymorphonuclear leukocyte in... Dependence may also be demonstrated by the phenomenon of 'satellitism ' now been identified haem! Privacy policy not be grown in the instructions for collection specimens such as urine and sputum are unsuitable... Decisiondatabases.Com for Global Transport Bags Market which Provides an in-depth analysis during the forecast period than salmonellas,:! From a clinical Specimen using the Gram stain enables the shape of bacterial cells be. > Campylobacters are now responsible for more cases of bacterial enteritis annually than salmonellas Establishing diagnostic microbiology laboratory -,! Methylene blue be confirmed following a positive culture result and then Transport a... Need to be explained in detail in the instructions for collection Management - shape of bacterial enteritis annually salmonellas. Is a more reliable test to differentiate staphylococci from streptococci than microscopic observation tubes for Salmonella spp but they seen! Microbiological testing are collection of the right bacterium to form spores but they are seen in clinical...., 2001 ), Establishing diagnostic microbiology laboratory - Chapter 06 - collection.
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Clostridium tetani Bacteria are gram-postive rods or bacilli with terminal spores that cause tetanus in humans, spore. Get complete Report @ https://www.marketresearchfuture.com/reports/electronic-toll-collection-market-4971, Future Market Insights Presents Swab and Viral Transport Medium Market Growth Projections in a Revised Study Based on COVID-19 Impact, - A recent FMI study shows the effect of COVID-19 on Swab and Viral Transport Medium Market: Global Industry Analysis and Review of Swab and Viral Transport Medium Market for 2020-2030, Swab and Viral Transport Medium Market to Witness Sales Slump in Near Term Due to COVID-19; Long-term Outlook Remains Positive.
$.' Mycobacteria Themycobacteria are a group of bacteria that are classified with other Gram-positive bacteria on the basis of their cellular architecture, but they possess a very waxy cell wall, and they rarely stain using conventional protocols such as the Gram stain. Ask the patient to hold the sputum container (already labeled) close to the lips and to spit into it gently after a productive cough. <>
Campylobacters are now responsible for more cases of bacterial enteritis annually than salmonellas. Various collection methods are available for specimens suspected of containing parasites or parasitic elements (Tables 4 to 7) (17). - Viral Transport Media. Viral updated Transport Bags Market Report 2019 Provides by Decisiondatabases.com. The image on the left is of Clostridium perfringens, stained from a clinical specimen using the Gram staining protocol. Various collection methods are available for specimens suspected of containing parasites or parasitic elements (Tables 4 to 7) (17). Even if you have a relatively good knowledge of the local flora, you may not be able to accurately identify all plants in the field. These differences have important consequences. Some microbiologists think that this gives them the microscopic appearance of Chinese letters. Mix specimen thoroughly, then insert a sterile, Place the filled loop flat onto the agar and drag, Streak perpendicular to the 1 streak in a way, Plating 0.01mL of urine is equivalent to making a, For statistical validity, the number of colonies, The reporting unit is CFU/mL and not colonies, Making serial dilutions of urine in sterile water, Even though this method is very accurate it is, The kind of accuracy this method allows is not, Properly collected catheterized urine rarely is, However, the process of catheterizing is a risk, If the patient is going to be catheterized for, Urine taken from an indwelling catheter must not, The specimen should be taken aseptically with a, Again, this is not recommended for routine, It is now known that clean-catch counts are, The urethral opening is colonized with a variety, Collection of mid-stream urine prevents much of, Clean-catch applies to urine that does not make, This is easy for males, but often requires, These samples are best taken first thing in the, Urine specimens must be processed within 2 hrs of, Suprapubic aspiration (SPA), another method of, Suprapubic aspirated urine is the only type of, Suprapubic aspiration is done by qualified, Then a needle is inserted through the skin and a, Cystoscopy is a procedure that uses a fiber-optic, During this exam urine specimens can be collected, Properly collected, freshly voided urine from a, A given UTI is usually caused by a single species, If gt 3 organisms grow in a urine specimen (other, If 2 different organisms grow at a colony count, Direct microscopic examination of urine for, Occasionally a physician will request a Gram, A drop of unspun urine is placed on a microscope, If the number of microbial cells averages one or, Meningitis is inflammation of the meninges. Specimen Collection and Transport The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). JFIF C - A new report avail by decisiondatabases.com for Global Transport Bags market which provides an in-depth analysis during the forecast period. Protect yourself and protect others Biosafety in specimen collection and transport - . Special handlingSome samples may require special handling, such as immediate refrigeration, protection from light or prompt delivery to the laboratory. The -haemolytic streptococci cause the complete breakdown of blood in fresh blood agar plates. sandy villar nca, ascp microbiology supervisor anmc. These are sometimes referred to as Dderlein bacilli. It appears that you have an ad-blocker running. Specimens such as urine and sputum are generally unsuitable for viruses isolation. The catalase test is a more reliable test to differentiate staphylococci from streptococci than microscopic observation. 
Spores of Gram-positive rods are highly resistant structures that may add considerably to their pathogenic capacity. dr ahmad mohammad ashshi (aibms) consultant virologist head of, Specimen collection in Infectious diseases - Specimen collection is an important part of microbial diagnosis of, Specimen Collection, Handling and Testing - To view the notes associated with this powerpoint presentation, click the. In culture, it is very difficult to persuade this bacterium to form spores but they are seen in clinical specimens. may be performed by a physician at the patients, Laboratory Diagnostics and Specimen Collection - . The family Enterobacteriaceae include Yersinia pestis, the cause of plague, Salmonella typhi, the cause of typhoid, Shigella dysenteriae, the cause of bacilliary dysentery, and Salmonella enteritidis implicated in many cases of food poisoning. Selective media for the isolation of Salmonella, Selective/differential media routinely used for, The differential agents in these media commonly, Colony color in the case of some media (EMB) is, There is a commercially available selective, Vibrio cholera and V. parahaemolyticus are not, Aeromonas and Pleisiomonas are also diarrhea, To aid in selection of the causative pathogens, Gram Negative (GN) and Selenite broths are two, It takes the normal gut bacteria six hours in GN, Salmonella in selenite, and Salmonella and, After several hours of incubation, the enrichment, Campylobacter jejuni and C. coli are thermophilic, Cefoperazone inhibit enteric Gram negative rods, Various strains of intestinal pathogenic E. coli, Enterohemorrhagic E. coli (EHEC) came to the, Many people became severely ill with painful, EHEC is a potentially deadly strain of E. coli, EHEC is detectable on a selective/differential, Commercially available ELISA tests can be used to, The LGH microbiology lab uses EHEC to report, Diarrhea that develops during a stay in the, Patients who develop diarrhea thee or more days, Nosocomial diarrhea is almost always associated, This antibiotic therapy eradicates a persons, Pseudomembranous colitis is characterized by the, Clostridium difficile is resistant to many, The most common method for detecting C. difficile, Sheeps blood agar will grow the infrequently, There are selective media available for each of, A major characteristic that differentiates, Colonies of oxidase positive Gram negative rods. Webthe desired tests at the place of collection or laboratory located far away, request the Diagnostic laboratories to advice on transportation of specimens, and consider how When to Request Transport Medium to preserve and transport in ideal medium before it is processed Popularly used transport medium are-Amie 's transport Medium: Because of the colour of halo that surrounds a-haemolytic streptococci, they are often referred to as "viridans" streptococci (viridis is Latin for green). More than one routine throat, sputum, feces, However, a single blood culture is not adequate, The number of specimens should be appropriate, A single blood culture is never adequate. at the end of the presentation, Specimen Transport and Management - . pathology, Biosafety: protect others, the environment. WebSpecimen Collection and Transport. specimens, Specimen Collection - . day 2. diagnostic examination. know the basic components of specimen, Laboratory Diagnostics and Specimen Collection - . 4. Create stunning presentation online in just 3 steps. materials for specimen collection. Webspecimen collection, preservation and transportation ppt dr. mervat, Establishing diagnostic microbiology laboratory - . Web Healthcare providers should educate patients on proper specimen production and collection Patients should also be informed of the possible infectious nature of his or her secretions Specimens should be collected in appropriate tubes that are sterile, clear, plastic, and leak-proof (50 ml screw capped centrifuge tubes Wipe off the obtained swab on the surface of the blood and make the inoculum . refer to nursing care guidelines 52-1. for shipement of speciemen or isolates, refer to the International Air Transport Association (IATA) Dangerous Goods Regulation (http://www.IATA.org/dangerousgoods/index), the U.S. Department of Transportation (http://hazmat.dot.gov/rules.htm) and the international Civil Aviation Organization (ICAO). They have retained the strong carbol fuchsin dye that has penetrated the cells following heating of the slide to steam heat, even though the film has been decolourised with a mixture of acid and alcohol. WebINTORDUCTION: Specimen collection and transportation are critical considerations , because any results the laboratory generates is limited by the quality of the specimen and its condition on arrival in the laboratory. The specimen must be delivered to the laboratory as soon as possible To overcome any delay and drawbacks due to delay in reaching the microbiology department, the following methods may be used. The background material has been counterstained with methylene blue. LM X500, Conventional Biochemical tubes for Shigella spp, Conventional Biochemical tubes for Salmonella spp. Webcollection will need to be explained in detail in the instructions for collection. specimen collection, preservation and transportation ppt. The X-factor has now been identified as haem, and the V-factor is nicotinamide adenine dinucleotide or NAD. Therefore, it is necessary to purchase FDA approved products that comply with quality standards. | PowerPoint PPT presentation | free to view It has millions of presentations already uploaded and available with 1,000s more being uploaded by its users every day. specimen collection. It cannot be grown in the absence of oxygen. Some bacteria are enclosed within a capsule. COLLECTION AND TRANSPORT the Gram stain enables the shape of bacterial cells to be observed easily. Webspecimen collection, preservation and transportation ppt. Here the diplococcal cells are seen within a polymorphonuclear leukocyte seen in pus from a urethral discharge. 3. Many of them are also animated. WINTER Refrigerate immediately at 4C, and then transport in a cold box. Successful laboratory investigations. For example, certain antibiotics cannot penetrate the outer membrane of Gram-negative bacteria, which are intrinsically resistant to these drugs as a consequence. WebView Chapter 06 - Specimen Collection and Handling.pptx.pdf from MLAB 2401 at Austin Community College District. Even if you have a relatively good knowledge of the local flora, you may not be able to accurately identify all plants in the field. Smears of expectorated sputum should be made. the most important aspects of microbiological testing are collection of the right. version 5.0, august 2012. objectives. laboratory tests are important in helping to diagnose patient problems. Urine Specimen Collection - . 3. Listeria monocytogenes is an important human pathogen, and it is capable of a characteristic tumbling motility seen at 25 degrees C but not at 37 degrees C. Lactobacilli appear microscopically as long, slender rods that often grow in chains. When collection methods are selected, the decision should be based on. Any important safety precautions should be explained. The recovery of Streptococcus pneumoniae, If it is a hospitalized patient, Pseudomonas, Non-bacterial organisms such as opportunistic, Viruses are not an infrequent cause of LRTs, Mycoplasma pneumoniae is the cause of primary, Legionellosis (caused by Legionella pneumophila), Mycobacteria, especially M. tuberculosis in the, Sheeps blood agar (SBA) is used for routine, S. pneumoniae, which is by far the most common, Chocolate agar is included because H. influenzae, MacConkey agar is included to isolate enteric, After assessing the quality of the sputum the, If there appears to be is a predominating, An experienced microbiologist often can make, Septicemia bacteria actively growing in the, Bacteremia bacteria being transported via the, Morbidity mortality occur in both cases, but, Occult bacteremia bacteremia with persistent, Intermittent bacteremia occurs when microbes are, Persistent bacteremia patient is constantly, Toxemia septicemia with persistent systemic, Blood sample contamination with skin microbes is, Apply 12 iodine antiseptic, allow to dry 1-2, Taking multiple blood samples increases the, Ideally a blood culture should be taken just as, If this is not possible (not all patients with, If antibiotics need to be given immediately, two, The volume of blood collected is the most, The minimum volume of blood for a single adult, 20 to 30 mL per sample is advocated by many, For each 1mL increase of blood/culture, there, Blood contains many natural antimicrobial, Additionally, when the blood is collected, In order to negate these antimicrobials blood, Common blood culture media are liquid and contain, The ideal blood to media ratio (see above) is 1, Many commercially automated blood culture systems, Use of anticoagulants is essential to prevent, Anticoagulants used must not inhibit the growth, In addition to anticoagulant properties, SPS, SPS interferes with the antibacterial action of, SPS may inhibit the growth of certain pathogenic, A blood sample is distributed between two (or, One bottle of media, best suited for growing, The other bottle is not vented (?CO2 ?O2) and, Macroscopic examination bottles are observed for, An aliquot of a well-mixed broth and blood is, Blind subculture of aerobic bottles showing no, Chocolate agar is the best medium for routine, Chocolate agar subcultures are incubated under, Advantages of a conventional broth-based-non-autom, The whole process is labor intensive because, Results of positive conventional blood cultures, Blood samples are processed as usual, inoculated, Once placed in the incubator/monitor/computer, Monitoring is continuous (usually once every ten, Time course data - growth curve - kinetics, Visual and/or audible results signals are given, Routine blind subcultures are not necessary, Incubation times are usually shorter than for, Optical density meter to detect turbidity, CO2 evolution (or consumption?) Specimen Collection and Labeling - . know the basic components of specimen collection, Specimen Collection, Handling and Testing - To view the notes associated with this powerpoint presentation, click the. Other Gram-negative bacilli Some Gram-negative bacilli appear so short that they resemble cocci in the light microscope. This is sufficient information to make a provisional diagnosis of gonorrhoea, which will be confirmed following a positive culture result. - Indirect drain collection method. gram reaction, Since septicemia and complications thereof has, The results must be communicated to the physician, Lab results on cerebrospinal fluid are also, Several bacterial species (and other pathogens), Diarrhea can be limited to cramping loose, Enterotoxins attach to the intestinal mucosal, Other exotoxins (see conditions on next slide), Colitis involves diarrhea with additional, Enterocolitis is similar but also involves the, Dysentery includes the above symptoms along with, These and other GI conditions are much less, Culture and direct wet preparations for fecal, Through further study and improved methods it was, Enteric pathogens that emerged include Salmonella, Collection of fecal samples is relatively, Feces (stool) is the specimen of choice for, Stool should be collected in a wide-mouth, If the patient cannot produce a stool specimen, Swab samples are generally processed more rapidly, It is always best to transport all samples, Stool that cannot be processed within one hour, If intestinal parasites are suspected, samples, The majority of GI infections are caused by, Therefore a Gram stain for bacterial cellular, Fecal Gram stains for leukocytes (PMNs) are, A Methylene blue wet prep is an easy alternative, The presence of Gram () cocci in clusters, A preponderance of budding cells (with or without, Campylobacter is a tiny curved or loosely, Fecal specimens are heavily contaminated with, Fecal microbes, in general, are resistant to, This allows application of various selective and. Note: On cysteine heart agar, F tularensis colonies are characteristically opalescent and do not discolor the medium (Dennis et al., 2001). Clipping is a handy way to collect important slides you want to go back to later. | PowerPoint PPT presentation | free to view heather nelson, rn. X and V dependence may also be demonstrated by the phenomenon of 'satellitism'. Microbiology 101 and Specimen Collection - . Webthe desired tests at the place of collection or laboratory located far away, request the Diagnostic laboratories to advice on transportation of specimens, and consider how When to Request Transport Medium to preserve and transport in ideal medium before it is processed Popularly used transport medium are-Amie 's transport Medium: WebVirology Specimen Collection Market Report 2022- Market Size, Trends, And Global Forecast 2022-2031 - TBRC global virology specimen collection market report includes blood collection kits, specimen collection tubes, viral transport media, swabs. 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